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. 2001 Jul 15;108(2):241–250. doi: 10.1172/JCI12020

Figure 1.

Figure 1

Normal (gray bars) and scleroderma (black bars) fibroblasts were grown to confluence on six-well plates. The experimental conditions were as follows: C, control with medium only; I, treated with 1 ng/ml Iloprost; T, treated with 10 ng/ml TGF-β; T+I, treated with 10 ng/ml TGF-β plus 1 ng/ml Iloprost added simultaneously. Media were removed after 24 hours for assay of (a) CTGF by ELISA, and (b) N-terminal propeptide of type I procollagen by ELISA. Values shown represent the means of six replicates of each experiment using cells derived from six patients with recent-onset diffuse scleroderma and from six healthy controls. *P < 0.05; **P < 0.001. (c) Normal human fibroblasts were grown to confluence and treated with 10 ng/ml TGF-β, with or without 1 ng/ml Iloprost and then lysed after 24 hours. CTGF was assayed by Western blot analysis. (d) In other experiments normal human fibroblasts were grown to confluence and treated with 10 ng/ml TGF-β with or without 1 ng/ml Iloprost. After 8 hours, the cells were lysed, and RNA was extracted for Northern blot analysis.