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Normal human fibroblasts were grown to confluence in six-well plates and treated at baseline with TGF-β (gray bars) (10 ng/ml). After 12 hours, Iloprost (1 ng/ml) was added to the media of some of the cells (black bars). (a) Media were removed at various time points for type I collagen assay using Western blot analysis. (b) Additional assays were performed on the media to measure CTGF by ELISA.
