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Normal human fibroblasts were grown to 50% confluence and infected with an adenovirus-encoding the cDNA for CTGF at 500 viral particles per cell. After 2 hours, the media were washed off and replaced with DMEM. (a) Subsequently, media were removed for assay of CTGF (gray bars) and type I collagen(black bars), by Western blot analysis. (b) The recombinant adenovirus encoding cDNA for human CTGF. LITR, left inverted terminal repeat; RITR, right inverted terminal repeat.
