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. 2001 Jul 15;108(2):241–250. doi: 10.1172/JCI12020

Figure 5.

Figure 5

(a) Normal fibroblasts were grown to confluence on six-well plates and treated with Iloprost (1 ng/ml) for various periods before lysis for cAMP measurement by enzyme immunoassay (gray bars). Other normal fibroblasts were treated with 2′,5′-DDA, an inhibitor of adenylate cyclase, for 2 hours before treatment with Iloprost (black bars). (b) Normal fibroblasts were treated with Iloprost at various concentrations for 30 minutes and then lysed for cAMP enzyme immunoassay (gray bars). Other normal fibroblasts were treated with TGF-β (10 ng/ml) for 2 hours before treatment with Iloprost, in order to determine whether Iloprost can elevate cAMP in the presence of TGF-β (black bars). (c) Normal fibroblasts were treated with TGF-β (10 ng/ml) with or without Iloprost (1 ng/ml) for 24 hours and before lysis for Western blot analysis for CTGF. Other normal fibroblasts were pretreated with 2′,5′-DDA for 2 hours before such treatment.