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. 2001 Jul 15;108(2):303–309. doi: 10.1172/JCI12517

Figure 1.

Figure 1

Hepatic and macrophage expression of human ABCA1 and plasma lipoprotein analysis in control and hABCA1-Tg mice. (a) Northern blot analysis of total RNA (20 μg) from representative control (C) and hABCA1-A Tg male mice. Human ABCA1 and β-actin cDNAs were utilized as probes (top and middle). Immunoblot analysis of mouse liver and macrophage homogenates (25 μg each) was performed using an anti-human ABCA1 polyclonal antibody (bottom). (b) Two-dimensional gel electrophoresis analysis of 15 μl of pooled plasma (n = 5; each group) from control and hABCA1-A Tg mice. (c) FPLC elution profile of pooled plasma (60 μl) from control (filled diamonds) and hABCA1-A Tg (open squares) mice. Ten microliters of FPLC fractions 1, 2, 3, and 4 was analyzed by immunoblotting as described in Methods (c, inset).