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. 2001 Jul 15;108(2):289–301. doi: 10.1172/JCI12539

Figure 1.

Figure 1

Scheme of the domain organization of the NHERF family and various constructs used in this study. (a) NHERF1 and NHERF2 contain two PDZ domains and a C-terminal ERM-binding region. PDZ domains of NHERF2 share 60–70% identity with each other and with the equivalent domains of NHERF1. A19 is the partial clone of NHERF2 initially obtained from yeast two-hybrid screening using PC tail as bait. (b) PDZ domains of NHERF proteins prepared by in vitro translation or expressed as FLAG-tagged fusion proteins. N1P1, first PDZ domain of NHERF1; N2P1, first PDZ domain of NHERF2; N1P2, second PDZ domain of NHERF1; N2P2, second PDZ domain of NHERF2.