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. 2001 Jul 15;108(2):289–301. doi: 10.1172/JCI12539

Figure 5.

Figure 5

Immunogold localization of NHERF2 in the glomerulus. (a) Double labeling for PC and NHERF2 in the rat glomerulus. PC (10 nm gold) and NHERF2 (5 nm gold) colocalize along the apical PM of foot processes (fp) above the slit diaphragms and along the cell membrane of the cell bodies of GECs (arrowheads). PC is also localized along the luminal surface of the glomerular endothelium (En), but NHERF2 is absent. No labeling for NHERF2 is seen at the base of the foot processes facing the GBM (B). (b, c, and d) Double labeling for ezrin and NHERF2 in rat glomeruli. Ezrin (10 nm gold) and NHERF2 (5 nm gold) are colocalized mainly along the apical PM of foot processes (arrowheads) but are not found along the basal surface of the foot processes below the level of the slit diaphragms. Ultrathin cryosections were doubly incubated with polyclonal anti-NHERF2 and either anti-PC (5A) or anti-ezrin (3C12) IgG followed by 5- or 10-nm gold goat anti-rabbit or anti-mouse IgG conjugates as described in Methods. Bars, 0.1 μm.