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. 1997 Apr 1;94(7):2951–2956. doi: 10.1073/pnas.94.7.2951

Figure 4.

Figure 4

Strand specificity of ssDNA intermediates. Wild-type λ (STU4)-infected strain FWS2520 (recJ) carrying pPAORM 3.8. Cells were harvested at time 0, 15 min, and 60 min into the infection. The series of blots presented in A–H are of total DNA extracts digested in vitro with PstI (expected DNA fragment sizes following in vitro PstI digestion: uncut modified phage DNA, 4749 bp; XhoI–PstI fragment to the right of the XhoI cut site, 3507 bp; and XhoI–PstI fragment to the left of the XhoI cut site, 1249 bp). Gels A–D examine events to the right of the XhoI cut site; gels E–H examine events to the left. A, C, E, and G are denatured blots; B, D, F, and H are native blots. A and B are hybridized with probe 250 (5′ specificity); C and D, with probe 249 (3′ specificity); E and F, with probe 54 (3′ specificity); and G and H, with probe 53 (5′ specificity). Lanes 1 and 5 in each gel are the size standards PstI/XhoI (3507 bp and 1249 bp) and PstI (4749 bp) (denatured native blot size standards were obtained by boiling the restricted DNAs immediately prior to electrophoresis); experimental samples are in lane 2 (time 0), lane 3 (15 min), and lane 4 (60 min).