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(A) EDL933; (B) EDL933ΔescN (T3SS-ATPase mutant); (C) EDL933ΔhcpA; (D) EDL933Δstx1,2; (E) EDL933ΔhcpAΔstx1,2; and (F) EDL933ΔhcpA(pJX22). HT-29 cell monolayers were incubated with these strains for 6 hours as described in Methods.
