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The indicated strains were incubated with human colonic Caco-2, T84, and HT-29 cells for 6 hours and the adherent bacteria expressed as CFUs after plating serial dilutions. The data are representative of at least 3 experiments performed in triplicate. Note the significant reduction in adherence in the EDL933ΔhcpA and EDL933ΔescN strains compared with the wild type to all the cell lines tested.
