Figure 5. CF cells show increased sensitivity to ExoA inhibition of protein synthesis.

CF and non-CF cells were incubated in media containing either increasing concentrations of ExoA for 24 hours (A) or 100 ng ExoA/ml (B) with IB3-1 and S9 (CFTR-corrected) cells. (C) CF primary lung epithelial cells in the presence or absence of furin inhibitor CMK (50 μM) or treated with 100 nM bafilomycin A₁ (BA). Protein synthesis levels were determined by measuring the incorporation of [3H] leucine into TCA-precipitable cellular proteins and are expressed as percentage of control cells that received no toxin. Mean ± SEM for 3 separate experiments; *P < 0.05, **P < 0.01, ^†P ≥ 0.05.