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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: Bone. 2007 Jul 13;41(4):535–542. doi: 10.1016/j.bone.2007.06.024

Figure 3. Ultrastructural localization of type XXVII collagen in the extracellular matrix.

Figure 3

Components of ECM were extracted from mouse growth plate cartilage and analyzed by immunogold transmission electron microscopy. Figures A and B show extracts that were dual-labeled with antibodies against type II collagen (12 nm particles) and type XXVII collagen (18 nm particles). Figures C–E show single label-staining with an antibody against type XXVII collagen (18 nm particles). (A) Type XXVII collagen was detected in fibrous, non-banded structures of width and length similar to that of banded collagen fibrils. These structures were devoid of type II collagen. (B) Large banded collagen fibrils showed heavy type II collagen staining and no type XXVII collagen labeling. (E) Type XXVII collagen was detected on some smaller banded fibrils. (C) Type XXVII collagen was detected in filamentous, amorphous mesh-works that were sometimes associated with the extracted collagen fibrils (D). Bar = 100 nm.

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