Skip to main content
. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Exp Eye Res. 2007 Mar 31;85(1):113–122. doi: 10.1016/j.exer.2007.03.007

Figure 3. Decreased Cx protein levels after AGA treatment.

Figure 3

Cells were pre-incubated with or without 200 μM AGA for 30 min followed by addition of 100 μM H2O2 for additional 3 h (total AGA treatment for 3 h 30 min). Whole cell extracts were isolated from PKCγ or C1B mutants (EGFP-tagged). Total protein levels of Cx43 and Cx50 are determined by Western blotting. α-tubulin is used as a loading control. The Western blot bands were digitized and relative Cx protein levels compared to α-tubulin were graphed. * indicates significant changes compared to levels in control cells with overexpression of wild type PKCγ.