Skip to main content
. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Exp Eye Res. 2007 Mar 31;85(1):113–122. doi: 10.1016/j.exer.2007.03.007

Figure 4. Lack of phosphorylation of Cx43 on Ser368 or Cx50 on serines by PKCγ C1B mutants.

Figure 4

A. Stably-transfected cells were treated with 100 μM H2O2 for 20 min. Phosphorylation of Cx43 on Ser368 was determined by Western blot using anti-phosphoSer368 Cx43 antibodies. α-tubulin levels were shown as controls. B. Cx50 was immunoprecipitated by anti-Cx50 antibodies. The immunoprecipitates were resolved by SDS-PAGE and immunoblotted with anti-phospho-serines antibodies (i.e., site of phosphorylation not presently identified). Cx50 levels were shown as controls.