TABLE 1.

Results from two-way ANOVA models for each outcome. The overall test (left column) shows results of testing for any difference in the outcome (Age-related or diet-related difference.) The next two columns show results of tests for differences across Age groups within LFD and HFD groups. The right-hand-side column shows results of testing for a difference between LFD and HFD diet groups aggregated over age. The numbers in parenthesis for each F-value are the numerator and denominator degrees of freedom associated with that particular test.

Outcome	Overall test	Age (LFD)	Age (HFD)	LFD vs. HFD
AgRP-adrenal	F(6,28) = 73.98	F(3,28) = 17.06	F(3,28) = 106.79	F(1,28) = 3.94
	p < 0.001	p < 0.001	p < 0.001	p = 0.057
AgRP-hypothalamus	F(6,28) = 6.59	F(3,28) = 5.86	F(3,28) = 9.41	F(1,28) = 4.39
	p < 0.001	p = 0.003	p = 0.002	p = 0.045
AgRP-testis	F(6,28) = 31.80	F(3,28) = 39.33	F(3,28) = 62.67	F(1,28) = 18.86
	p < 0.001	p < 0.001	p < 0.001	p < 0.001
Leptin	F(6,28) = 34.40	F(3,28) = 43.90	F(3,28) = 50.19	F(1,28) = 54.89
	p < 0.001	p < 0.001	p < 0.001	p < 0.001
NPY	F(6,28) = 7.04	F(3,28) = 10.87	F(3,28) = 4.35	F(1,28) = 0.48
	p < 0.001	p < 0.001	p = 0.012	p = 0.0493
OBRb	F(6,28) = 3.43	F(3,28) = 1.58	F(3,28) = 4.87	F(1,28) = 0.01
	p = 0.001	p = 0.217	p = 0.008	p = 0.928