Table 4.
Relative motion among three α-helices in insulin and its analogs
| Molecule | A1–A8 | A12–A18 |
|---|---|---|
| DHPI(1) | ||
| θ, ° | 0 | 0 |
| t, Å | 0 | 0 |
| DHPI(2) | ||
| θ, ° | 5.20 | 5.82 |
| t, Å | 2.07 | 2.41 |
| INS | ||
| θ, ° | 2.24 | 6.04 |
| t, Å | 0.99 | 3.56 |
| DPI | ||
| θ, ° | 7.20 | 7.14 |
| t, Å | 3.40 | 3.04 |
| DHI | ||
| θ, ° | 5.99 | 5.72 |
| t, Å | 1.90 | 1.57 |
| DesB1-B2DPI | ||
| θ, ° | 5.85 | 3.49 |
| t, Å | 2.68 | 0.79 |
Based on the main chain superposition of insulin and insulin analogs in α-helix B9–B19, the rotation (θ) and translation (t) of the other two α-helices relative to B9–B19 of DHPI(1) are computed by superposition facility in x-plor (26). DesB1–B2DPI, des(B1–B2) despenta peptide (B26–B30) insulin. INS represents 2Zn insulin molecule 1, while DHPI(1) and DHPI(2) represent the DHPI molecules 1 and 2, respectively.