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Gene targeting vectors, disruption strategy, and genotyping. (A) Genomic organization of Plcg1 with exons indicated by solid boxes. Also depicted are the targeting vectors TV-I and TV-II and the disrupted Plcg1 alleles for each targeting vector. B, BamHI; R1, EcoRI; K, KpnI. (B and C) Southern blots identifying wild-type and disrupted Plcg1 alleles after TV-I targeting of ES cells or embryos, respectively. (D) Representative PCR genotyping of Plcg1 alleles from embryos.
