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. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Int J Oncol. 2007 Nov;31(5):1039–1050.

Figure 1. RNAi-mediated downregulation of cathepsin B and MMP-9 expression in IOMM-Lee cells.

Figure 1

(A) Total RNA was extracted from IOMM-Lee parental cells (mock) and cells transfected with pSV, pCB, pM and pMC. Semi-quantitative RT-PCR was performed as per standard protocol. GAPDH expression was also analyzed in all the samples to serve as a loading control. (B) Cell lysates were collected from cells transfected with pSV, pCB, pM and pMC and also from parental cells (mock). Immunoblot analysis was performed using the cell lysates to detect expression of cathepsin B in the parental cells and the cells transfected with shRNA plasmids. GAPDH was analyzed as a loading control. (C) Enzymatic activity of MMP-9 was analyzed in the conditioned media collected from cells transfected with pSV, pCB, pM, pMC and parental cells. 30 μg of protein were loaded onto 10% SDS-PAGE containing gelatin.