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. Author manuscript; available in PMC: 2007 Oct 16.
Published in final edited form as: Biochemistry. 2006 Aug 8;45(31):9632–9638. doi: 10.1021/bi060754b

Table 1.

Characterization of TyrH Mutant Enzymesa

enzyme Ktyrb
(μM)		 K6MePH4c
(μM)		 kcat
(min−1)
wild-type TyrH  80 ± 18 45 ± 4 182 ± 26
W372F  8 ± 1 45 ± 5 44 ± 3
W166F/W372F 90 ± 4 180 ± 18 243 ± 5 
W166F/W233F/W372F 90 ± 6 162 ± 15 276 ± 7 
F184W/W372F 17 ± 5 31 ± 4 64 ± 6
W166F/F184W/W372F 65 ± 5 129 ± 4  184 ± 5 
F3W 64 ± 6 116 ± 11 165 ± 5 
a

Conditions: 0.2 μM enzyme, 100 μg/mL catalase, 50 mM HEPES at pH 7.0, 10 μM ferrous ammonium sulfate, and 1 mM DTT at 25 °C.

b

6MePH4 (380 μM) with varied concentrations of tyrosine.

c

Tyrosine (100 μM) with varied concentrations of 6MePH4.