. Author manuscript; available in PMC: 2007 Oct 16.

Published in final edited form as: Biochemistry. 2006 Aug 8;45(31):9632–9638. doi: 10.1021/bi060754b

Table 2.

Effects of Ligands on the Fluorescence Lifetimes of the Tryptophan in F₃W TyrH

additions	C (ns)^a	W (ns)^a
none	4.47 ± 0.04	2.53 ± 0.06
phenylalanine (10 mM)	4.24 ± 0.04	2.81 ± 0.06
6-methyl-5-deaza-tetrahydropterin (500 μM)	4.38 ± 0.04	2.90 ± 0.06
6-methyl-5-deaza-tetrahydropterin (500 μM) plus phenylalanine (10 mM)	4.13 ± 0.05	3.01 ± 0.07

From a fit of the data to eq 3.