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. Author manuscript; available in PMC: 2007 Oct 16.
Published in final edited form as: Biochemistry. 2006 Aug 8;45(31):9632–9638. doi: 10.1021/bi060754b

Table 3.

Effects of Ligands on the Pre-exponential Amplitude and Cone Angle of the Tryptophan in F3W TyrH

additions r0–r∞a 2ϕ (deg)a
none 0.048 ± 0.014 46 ± 3
phenylalanine (10 mM) 0.041 ± 0.015 42 ± 2
6-methyl-5-deaza-tetrahydropterin (500 μM) 0.027 ± 0.014 34 ± 2
6-methyl-5-deaza-tetrahydropterin (500 μM) plus phenylalanine (10 mM) 0.028 ± 0.015 34 ± 2
a

Differential polarization data were fit to a model describing a hindered rotation with a slow global rotation using eq 2. The cone angle of the local rotation for the tryptophan was obtained from eq 5.