Conserved sequence patches in the vicinity of the gatae gene, and their cis-regulatory activities. (A), FamilyRelations analyses of S. purpuratus and L. variegatus gatae BACs. A 50 bp window was applied in the analysis, with an 85% identity threshold. Almost the same results were obtained using a 92% identity threshold, except for the disappearance of a few patches that were inactive in the experiments of part (B). Exons are represented by blue boxes, and the two active cis-regulatory modules are shown in green. (B), Diagrammatic representation of constructs created in gatae cis-regulatory analysis, and summary of activity. At the top is a map of the conserved sequence patches (red boxes) from the analysis in (A). The reporter constructs tested in this work are shown below. The name of each construct is listed in the left column, and an indication of its activity over background is at the right. Here purple boxes denote the conserved sequence patch containing the gatae basal promoter, and green boxes represent the GFP coding region.