Figure 2.

(A) Northern blot analysis of E3KARP and NHERF expression in rabbit ileal villus cells, PS120, and Caco-2 cells. Two micrograms of poly(A)⁺ enriched RNA from either PS120 fibroblasts or Caco-2 cells was electrophoresed per lane. For the rabbit ileal villus cells, 30 μg of total RNA was used. Hybridization was done under high-stringency conditions to avoid crosshybridization between E3KARP and NHERF. (B) Western blot analysis of expression of NHERF. Lanes: 1, brush border membrane from rabbit kidney and crude membranes from 2, CHO; 3, OK; 4, PS120; and 5, LLC-PK₁ cells. All lanes were loaded with 15 μg of membranes and were probed with polyclonal antibody against NHERF.