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Association of Cdc2 with the centrosome in Saos-2 human osteosarcoma cells. (Left) Immunostaining was carried out using Cdc2 mouse monoclonal antibody (red) and a pericentrin rabbit polyclonal antibody (green) on detergent extracted cells (Upper) or NM-IF preparations (Lower). The colocalized signals are yellow. (Right) DAPI staining shows presence of DNA in nuclei of detergent-extracted cells (Upper) but not in NM-IF preparations (Lower).
