Figure 1.

Activation of Ras in CEF and Rat-2 cells expressing v-Src or SRX5. CEF (open bars) were infected with vector control, v-Src, or SRX5 virus 2 days before serum starvation. The Rat-2 cell lines (solid bars) fpGV1-1-1 (vector control), v-Src-A4, and SRX5-C20 were plated 24 hr before serum starvation. The activation state (% GTP-bound) of endogenous Ras was assayed as described and is expressed relative to the level observed in vector control cells. The data are expressed as the mean ± SEM of three independent experiments.