Figure 3.

Effect of N17Ras expression on morphological transformation of CEF and Rat-2 cells. (A–C) Morphology of CEF that were infected with empty vector viruses (A), empty vector subgroup B and v-src subgroup A viruses (B), or N17ras subgroup B and v-src subgroup A viruses (C). (D–F) Morphology of Rat-2 cell lines that were grown in serum-containing medium in the presence of 100 μM ZnCl₂ and 2 μM CdCl₂ for 24 hr: (D) Rat-2/pM2N-1, (E) Rat-2/pM2NSrc, and (F) Rat-2/N17RasSrc (see Fig. 2B legend for description of cell lines). Images were acquired using a Zeiss Axiovert microscope with a ×20 objective.