Table 1.

Effect of dominant-negative Ras on transformation of CEF by v-Src

Cells*	Src virus†	Focus formation,‡ %	Soft agar colony formation,‡ %	Hexose uptake,§ %
CEF/Vec	−	0	0	100 ± 9
CEF/Vec	+	100 ± 3	100 ± 10	536 ± 85
CEF/N17Ras	−	0	0	59 ± 5
CEF/N17Ras	+	110 ± 16	144 ± 23	414 ± 51

The data are expressed as the mean ± SEM (percent of control) of three replicate plates in a single experiment, and are representative of two to four independent experiments. 

^*CEF/Vec, cells that were infected with the subgroup B empty vector virus; CEF/N17Ras, cells that were infected with the subgroup B N17Ras virus. 

^†Cells were infected with the subgroup B virus 2 days before superinfection with the subgroup A empty vector (−) or v-Src (+) virus. 

^‡Cells were infected with the subgroup A virus at a low multiplicity of infection (<1) for focus and soft agar colony assays. 

^§Cells were infected with the subgroup A virus at a high multiplicity of infection (≈10) for the hexose uptake assay.