Figure 3.

Nuclear APC protein co-localizes with rRNA. 184A1 cells were stained simultaneously for APC protein (a) and rRNA (b) using antibodies specific for the APC protein and a fluorescein isothiocyanate-conjugated DNA oligo complementary to rRNA. The yellow staining in c reflects the colocalization of rRNA and APC protein. Solid arrowheads designate nuclear staining in a single cell (a–c). Open arrowheads designate additional regions of staining overlap. d is a DIC and DAPI view of the fluorescence views shown in a–c. (Bar = 10 μm.)