Figure 5.

Truncated APC protein does not fractionate with the nucleus of DLD-1 cells. Proteins within the various fractions of DLD-1 cells were analyzed by SDS/PAGE and immunoblotting. Fractions are labeled as follows: T, total; C, cytoplasm; M, membrane/cytoskeleton; N, nucleus; Sc, nuclear scaffold. The antibodies used for the Western immunoblots include: a, APC; b, tubulin as a cytoskeletal marker; c, β-adaptin as a membrane marker; and d, lamins as nuclear and nuclear matrix scaffold markers. Truncated APC protein is present in the membrane/cytoskeletal fraction, but not the nuclear or nuclear scaffold fractions.