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Intranuclear localization of Ro RNA in skin by in situ hybridization with hY1 + hY3 + hY4 + hY5 probe mixture. A cluster of gold particles (10 nm; arrow) is located at the periphery of the nucleolus (nu) in association with a portion of its fibrillar component, whereas individual gold particles are scattered through the cell. Formaldehyde fixation was 4%. Image produced by uranyl acetate staining. (Bar = 0.5 μm.)
