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. Author manuscript; available in PMC: 2008 Aug 24.
Published in final edited form as: J Mol Biol. 2007 Jun 2;371(4):971–988. doi: 10.1016/j.jmb.2007.05.075

Figure 7.

Figure 7

The salt dependence of the free energy of dissociation for the H2A-H2B dimers from the NCP, showing extrapolations to physiological ionic strengths. A) H3-78W/H2A-108Cys-AEDANS NCP. Values were calculated from FL intensities at 350 nm (●, solid lines) and 490 nm (○, dashed lines) and were fit to two lines which intersect near the transition midpoint (Figure 6). Inset: overlay of data from the three H2A-108Cys-AEDANS NCPs; the lines represent the average ΔG°(H2O) and m values given in the text. B. H2B-109Cys-AEDANS NCPs with H3-78W (●, solid lines) or H4-60W (■, dashed lines). Conditions are given in legend of Figure 5.