Table 4.

Oligonucleotide primers used in the N. gonorrhoeae MLST scheme

Locus	Name	Sequence (5'-3')	Function
abcZ	abcZ-P1	AATCGTTTATGTACCGCAGG	Amplification and sequencing
abcZ	abcZ-S2	GAGAACGAGCCGGGATAGGA	Amplification and sequencing
adk	adk-P1	ATGGCAGTTTGTGCAGTTGG	Amplification
adk	adk-P2	GATTTAAACAGCGATTGCCC	Amplification
adk	adk-S1	AGGCTGGCACGCCCTTGG	Sequencing
adk	adk-S2	CAATACTTCGGCTTTCACGG	Sequencing
aroE	aroE-P1	ACGCATTTGCGCCGACATC	Amplification and sequencing
aroE	aroE-P2	ATCAGGGCTTTTTTCAGGTT	Amplification
aroE	aroE-S2	ATGATGTTGCCGTACACATA	Sequencing
fumC	fumC-P1	CACCGAACACGACACGATGG	Amplification
fumC	fumC-P2	ACGACCAGTTCGTCAAACTC	Amplification
fumC	fumC-S1	TCCGGCTTGCCGTTTGTCAG	Sequencing
fumC	fumC-S2	TTGTAGGCGGTTTTGGCGAC	Sequencing
gdh	gdh-P1	ATCAATACCGATGTGGCGCGT	Amplification
gdh	gdh-P2	GGTTTTCATCTGCGTATAGAG	Amplification and sequencing
gdh	gdh-S3	CCTTGGCAAAGAAAGCCTGC	Sequencing
pdhC	pdhC-P1	GGTTTCCAACGTATCGGCGAC	Amplification
pdhC	pdhC-P2	ATCGGCTTTGATGCCGTATTT	Amplification and sequencing
pdhC	pdhC-S1	TCTACTACATCACCCTGATG	Sequencing
pgm	pgm-S1	CGGCGATGCCGACCGCTTGG	Amplification and sequencing
pgm	pgm-S2	GGTGATGATTTCGGTTGCGCC	Amplification and sequencing

Oligonucleotide primers were as previously published [16,25].