Figure 2.

Transient expression reveals AtCNGC10-GFP trafficking to the plasma membrane of Arabidopsis leaf protoplasts using confocal laser scanning microscopy. (A) Leaf protoplasts were transfected with the two constructs pBL-35S:AtCNGC10-EGFP (C10-GFP) and pBL-35S:EGFP alone (GFP), and compared with untransfected controls (WT). Images were acquired through laser scanning confocal microscopy and include: (g) a 505–525 nm emission filter for GFP, (r) chlorophyll autofluorescence detected using a 650 nm emission filter, and (rg) merged GFP and chlorophyll autofluorescence. (B) Solid white arrowheads label AtCNGC10-GFP in punctate regions, while open arrowheads label vesicle-like structures.