Table 3.
Examples of genes showing differential expression in longissimus muscle of bovine fetuses from different sire breeds in at least one time point1
| Gene2 | GenBank Accession3 | Number of array elements4 | Gene expression ratio5 P60d/W60d | Gene expression ratio5 P135d/W135d | Gene expression ratio5 P195d/W195d | Gene expression ratio5 Pbirth/Wbirth |
| Aldolase A, fructose-biphosphate (ALDOA) | CF615006 | 4 | 0.87 | 1.04 | 2.16** | 0.90 |
| Collagens (COL1A1, COL1A2, COL3A1, COL12A1, COL15A1) | Additional file 2 | 42 | 1.21 | 0.70 | 0.96 | 0.64** |
| Cytochrome c oxidases (COX5B, 6C, 7B, 7C) | Additional file 2 | 9 | 0.93 | 0.99 | 0.84 | 1.74** |
| Fatty acid binding protein 4, adipocyte (FABP4) | CF614083 | 3 | 1.01 | 0.70** | 0.90 | 0.98 |
| Fatty acid binding protein 5 (FABP5) | CF613827 | 1 | 1.29 | 0.93 | 1.16 | 0.44** |
| Matrin 3 (MATR3) | CF614914 | 1 | 2.35** | 1.15 | 0.79 | 0.64** |
| Ribosomal L proteins (RPL) | Additional file 2 | 32 | 0.75 | 0.93 | 1.01 | 1.52** |
| Ribosomal S proteins (RPS) | Additional file 2 | 13 | 0.80 | 0.89 | 0.99 | 1.51** |
1* indicates a statistically significant gene expression difference at P < 0.05 level; ** indicates a statistically significant gene expression difference at P < 0.01 level.
2Sequence annotation was carried out using approved gene symbols (human genome nomenclature) via the IBISS4 database [17]. BLAST [15] scores > 110 and e-values < 2 E-23 were accepted for annotation purposes.
3in cases where more than 1 microarray element was used to calculate the expression data, the accession number of 1 representative element is shown.
4Number of microarray elements representing the same gene that were used to calculate the expression data.
5To obtain the ratios shown, averaged absolute microarray signal intensity values for P (Piedmontese × Hereford) fetuses were divided by the signal intensities measured for W (Wagyu × Hereford) fetuses at d 60, d 135, d 195 and birth.