Table 5.

Quantitative reverse transcription PCR (qRT-PCR) measurements of gene expression in total RNA from longissimus muscle of bovine fetuses – gene expression ratios between breeds

Gene	Fold Ratio1
	P60d/W60d	P135d/W135d	P195d/W195d	Pbirth/Wbirth
Myostatin (GDF8)	1.52 **	1.45 *	1.15	1.51 **
Fatty-acid binding protein 4 (FABP4)	1.32	0.56	0.14 **	0.20 **
Fatty-acid binding protein 5 (FABP5)	0.88	1.09	1.00	0.54 **
Matrin 3 (MATR3)	1.10	1.10	0.96	0.75

¹To obtain fold ratios, mean normalized (from ANOVA and against 18S RNA) qRT-PCR values from P (Piedmontese × Hereford) samples were divided by those from W (Wagyu × Hereford) samples at 60 d gestation, 135 d gestation, 195 d gestation and birth.

* Significance at P < 0.05; ** Significance at P < 0.01.