Figure 1.

IL-8 stimulated activation of ERK but not p38-MAPK is sensitive to wortmannin and PD098059. Human neutrophils were treated with either the carrier dimethyl sulfoxide alone, 100 nM wortmannin for 10 min or 50 μM PD098059 for 1 hr prior to stimulation with 25 nM IL-8. (A) Samples were stimulated for 3 min then assayed for ERK activity. The data shown represent the mean ± SEM of triplicate measurements of ERK activity for each sample and are representative of more than six independent experiments. ∗, P = 0.0126; ∗∗, P = 0.0066; ∗∗∗, P = 0.0003. (B) Samples were stimulated for 10 min then assayed for p38-MAPK activity. The data shown represent the mean ± SEM of PhosphorImager data from five independent experiments.