Table 3.
Horizontal transmission of LGTV from I. scapularis larvae to C57Bl/6 mice
| Moribund mice | Survivors | ||||
|---|---|---|---|---|---|
| Treatmenta | Placementb (dpim) |
% Mortality
(# dead / total mice)c |
Viral RNA
detected in brains d |
% Seroconversion
(# seroconverted / # survivors) |
Viral RNA
detected in brainsd |
| Mock | 17 | 0% (0/8) | - | 0% (0/8) | - |
| Infected | 3 | 37.5% (3/8) | + | 100% (5/5) | + |
| Infected | 5 | 12.5% (1/8) | + | 100% (7/7) | + |
| Infected | 10 | 75% (6/8) | + | 100% (2/2) | + |
| Infected | 17 | 50 % (4/8) | + | 100% (4/4) | + |
a
Ticks were immersed in media alone (Mock) or in media containing LGTV (Infected)
b
Ticks were placed on mice at 3, 5, 10 and 17 dpim.
c
Mice were considered terminal at the first signs of neurological disease.
d
Total RNA was isolated from the brains of mice and detection of viral RNA is represented by (+) presence, or (-) absence.