Figure 3.

(A) Structure of the TIE2 locus, including the first exon (shaded box), flanked by upstream and intronic sequences [open box, XhoI–KpnI fragment (XK); solid box, NcoI–XbaI fragment (NcXb)]. (B) Structure of LacZ reporter gene constructs and their respective relative activities (HH− = 100%) in transient transfections of BAECs. Values significantly (P < 0.05; Student’s t test) above 100% have been marked with an asterisk. (C) Activity (in percent of tk core promoter activity) of HH− and HHXK in BAECs and HEK293 cells. (D) Activity of tkXK (tk promoter and 1.7-kb enhancer) and tkNcXb (tk promoter and 303-bp enhancer fragment) in BAECs.