Table 2.
Summary of the activity of different mutated constructs in transgenic embryos at E11.5
| Construct (mutated sites) | Wild-type sequence | Mutant sequence | TG | ES |
|---|---|---|---|---|
| tkXK | Wild-type control | No mutations | 13 | 6 |
| tkXK (GATA+αIFN2) | ..AAGTGATAGAAATGA.. | ..AAcgcgtATGA.. | 12 | 7 |
| tkXK (Sp1) | ..GAGGCTGAT.. | ..GAcgcgtAT.. | 12 | 6 |
| tkXK (∗∗∗+NS-F) | ..CAGATCTGTCAGC.. | ..CAGAcgcgtGC.. | 13 | 4* |
| tkXK (Ets1) | ..GCTTCCTG.. | ..GCacgcgTG.. | 9 | 0 |
| tkXK Δ(bZIP, CP2-γ, PEA3) | Deletion of the SacI–XbaI region | 18 | 0 | |
Abbreviations are as in Fig. 1; ∗∗∗, octameric palindrome. Data are presented as numbers of embryos.
*
Endothelial staining was reduced or enhanced ectopic staining was observed.