Figure 5.

Timing of the late rapamycin-sensitive step during late meiosis. Diploid SK-1 cells were incubated in YPD for 24 hr at 30°C with vigorous shaking. These cells were then diluted 1:50 into YPA. After 13.5 hr of further incubation, they were washed twice with sterile water and resuspended into SPM (0 hr), and then 100 nM rapamycin was added to various culture aliquots at 0, 2, 4, 6, 8, and 10 hr, and the proportion of different asci in each sample was analyzed as in Fig. 3a.