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. 1989 Dec;55(12):3226–3228. doi: 10.1128/aem.55.12.3226-3228.1989

Enumeration of Enterobacter cloacae after chloramine exposure.

S K Watters 1, B H Pyle 1, M W LeChevallier 1, G A McFeters 1
PMCID: PMC203251  PMID: 2619309

Abstract

Growth of Enterobacter cloacae on various media was compared after disinfection. This was done to examine the effects of monochloramine and chlorine on the enumeration of coliforms. The media used were TLY (nonselective; 5.5% tryptic soy broth, 0.3% yeast extract, 1.0% lactose, and 1.5% Bacto-Agar), m-T7 (selective; developed to recover injured coliforms), m-Endo (selective; contains sodium sulfite), TLYS (TLY with sodium sulfite), and m-T7S (m-T7 with sodium sulfite). Sodium sulfite in any medium improved the recovery of chloramine-treated E. cloacae. However, sodium sulfite in TLYS and m-T7S did not significantly improve the detection of chlorine-treated E. cloacae, and m-Endo was the least effective medium for recovering chlorinated bacteria. Differences in recovery of chlorine- and chloramine-treated E. cloacae are consistent with mechanistic differences between the disinfectants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braswell J. R., Hoadley A. W. Recovery of Escherichia coli from chlorinated secondary sewage. Appl Microbiol. 1974 Aug;28(2):328–329. doi: 10.1128/am.28.2.328-329.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Camper A. K., McFeters G. A. Chlorine injury and the enumeration of waterborne coliform bacteria. Appl Environ Microbiol. 1979 Mar;37(3):633–641. doi: 10.1128/aem.37.3.633-641.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hoadley A. W., Cheng C. M. The recovery of indicator bacteria on selective media. J Appl Bacteriol. 1974 Mar;37(1):45–57. doi: 10.1111/j.1365-2672.1974.tb00413.x. [DOI] [PubMed] [Google Scholar]
  4. LeChevallier M. W., Cameron S. C., McFeters G. A. New medium for improved recovery of coliform bacteria from drinking water. Appl Environ Microbiol. 1983 Feb;45(2):484–492. doi: 10.1128/aem.45.2.484-492.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LeChevallier M. W., Cawthon C. D., Lee R. G. Inactivation of biofilm bacteria. Appl Environ Microbiol. 1988 Oct;54(10):2492–2499. doi: 10.1128/aem.54.10.2492-2499.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lin S. Evaluation of coliform tests for chlorinated secondary effluents. J Water Pollut Control Fed. 1973 Mar;45(1):498–506. [PubMed] [Google Scholar]
  7. McFeters G. A., Camper A. K. Enumeration of indicator bacteria exposed to chlorine. Adv Appl Microbiol. 1983;29:177–193. doi: 10.1016/s0065-2164(08)70357-5. [DOI] [PubMed] [Google Scholar]
  8. McFeters G. A., Kippin J. S., LeChevallier M. W. Injured coliforms in drinking water. Appl Environ Microbiol. 1986 Jan;51(1):1–5. doi: 10.1128/aem.51.1.1-5.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rice E. W., Fox K. R., Nash H. D., Read E. J., Smith A. P. Comparison of media for recovery of total coliform bacteria from chemically treated water. Appl Environ Microbiol. 1987 Jul;53(7):1571–1573. doi: 10.1128/aem.53.7.1571-1573.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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