Figure 2.

In situ hybridization of mouse embryonic brain demonstrates HFH-4 expression in the choroid plexus epithelium. In situ hybridization of transverse sections of embryonic head region with ³³P-labeled antisense HFH-4 RNA probe. (A) HFH-4 expression in 14.5 day p.c. mouse embryonic brain. HFH-4 expression is found in the choroid plexus (CP) epithelium and ependymal cells of the fourth ventricle (FV) and lateral ventricle (LV). (B) HFH-4 expression in 13.5 day p.c. mouse embryonic brain. HFH-4 expression is found in the choroid plexus epithelium and ependymal cells of the fourth ventricle but not in the nasal cavity (NC) epithelium or the lens (L) and epithelium of the eye. (C) HFH-4 expression in 12.5 day p.c. mouse embryonic brain. HFH-4 expression is found in the choroid plexus epithelium of the fourth, lateral, and third ventricle (ThV or mesencephalic vesicle) of the brain.