Figure 3.

Identification of HFH-4 transcriptional activation protein motifs. Schematically shown is the position of potential HFH-4 transcriptional activation motifs of the wild-type HFH-4 protein (5) and truncated HFH-4 proteins synthesized by deleted HFH-4 cDNA constructs. Numbers represent the amino acid positions of the potential transcriptional activation motifs and the winged helix DNA-binding domain. Summarized in the bar graph is the normalized transcriptional activity of the HFH-4 protein deletions relative to the wild-type activity and the error bars represent the SD from three separate experiments. Western blots using an HFH-4 antibody specific to the N terminus of the protein (amino acids 1–100) showed that HFH-4 protein was expressed in nuclear extracts (data not shown).