Table 2.

HFH-4 binding  activity

Binding site	Sequence	HFH-4 Binding*
1. HFH-8	ATGTGTTTGTTTA	+++
2. HNF-3 no. 4	CAATGTTTGTTTA	+++
3. HFH-2 no. 7	gTTTGTTGTTTTA	+++
4. HFH-2 no. 12	TATTGTTaTTTTA	+
5. HFH-4 no. 3	gTTTGTTTacaat	++
6. HNF-3 no. 4m1a	CAATaTTTGTTTA	−
7. HNF-3 no. 4m2a	CAATaTTTaTTTA	−
8. Ceruloplasmin	CAGaGTTTGTgTg	−
9. TTF-1	TcGcGTTTGTTTt	−
HFH-4 Consensus:	HWDTGTTTGTTTA

Isolation of HFH-2 and HNF-3 DNA binding sequences was previously (24). Nucleotides which deviate from the HFH-4 consensus or HFH-2 no. 7 sequence are indicated in lowercase letters. Nucleotide abbreviations are as follows: H is not G, W is A or T and D is not C. Ceruloplasmin, ceruloplasin −245/−233; TTF-1, thyroid transcription factor-1 −196/−208. 

^*HFH-4 protein binding affinity is as follows: +++, high; ++, moderate; +, weak; −, none.