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. 2003 Sep 15;31(18):5368–5376. doi: 10.1093/nar/gkg706

Figure 7.

Figure 7

Sp3 show no synergistic transactivation of promoters containing multiple Sp-binding sites. (A and B) The indicated reporter plasmids (5 µg) were transfected into Drosophila SL2 cells with various amounts of the Sp1 expression plasmid (pPacSp1) and Sp3 expression plasmid (pPacSp3). The cells were subsequently lysed, assayed for CAT activity and the values represent the fold induction of each reporter plasmid calculated from three independent experiments. (C) The CAT reporter plasmid BCAT-2 (2.5 µg) was transfected into Drosophila SL2 cells along with Sp1 expression plasmid pPacSp1 (50 ng), and varying levels of Sp3 expression plasmid pPacSp3 (25 ng to 1 µg). The cells were subsequently lysed and assayed for CAT activity. The values represent the average of three independent experiments. (D) Comparable expression of Sp3 and Sp1 in transfected Drosophila cells. The pPacSp1 (5 µg) and pPacSp3 (5 µg) were transfected into Drosophila SL2 cells. The cells were subsequently lysed, and 50, 100 and 200 µg of cell extracts were analyzed for the Sp1 and Sp3 protein using western blot assay.