Figure 5.

Western blot analysis of lymphoblast extracts probed with antibodies to XPG, TAF_II130, and XPB. The lymphoblast lines XPG82 (lane 82) and XPG83 (lane 83) are derived from patients XP124LO and XP125LO, respectively; XPG81 (lane 81) is from their obligate heterozygote mother. DS2 is a wild-type lymphoblast line (40). Note that the XPG protein, which has a predicted molecular mass of 133 kDa, is present in the two XP-G patients, but in reduced amounts, and that it migrates very close to the 205-kDa myosin marker.