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(A) Representation of the strategy used to detect the orientation of the 71 kb inversion. The P.patens (top), M.polymorpha and A.formosae (bottom) genomes are shown in the region of the 71 kb inversion. The positions of primers P1–P4, located adjacent to the inversion endpoints, are indicated. (B) DNA regions amplified with either the P1 and P2 pair or the P1 and P3 pair are illustrated, with the length (bp) of the intergenic spacer.
