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. 2003 Sep 15;31(18):5238–5246. doi: 10.1093/nar/gkg747

Figure 5.

Figure 5

Specific transactivation and binding competition of CDX proteins on the Glc6Pase promoter. (A) Amino acid sequence of the homeodomains of the murine and human CDX1 and CDX2 proteins. Uppercase letters indicate the differences between these proteins. (B) HepG2 cells were transiently transfected with the –35/+60B construct (1 µg) together with pCMV-RL (2 ng, used as internal control), without or with 100 ng of pCDX1-S or pCDX2-S, or pCDX1HD2, or pCDX2HD1. (C) HepG2 cells were transiently transfected with the –35/+60B construct (1 µg) together with pCMV-RL (2 ng), in the presence of pCDX1-S (100 ng) and pCDX2-S (1–200 ng, black circles) or pCDX2-HD1 (1–200 ng, black triangles), or pCDX1-HD2 (1–200 ng, black squares) expression vectors. LUC activities were determined 48 h after transfection and were normalized with regards to the level of RL activities. The transactivation rate is expressed as fold of induction over the basal condition. Results are the mean ± S.E.M. of at least three independent experiments performed in duplicate.*, significantly different from without CDX, P < 0.01.