Figure 6.

Growth rate comparison of qkgA^– and parental Ax-2 cells. (A) Cells growing in HL-5 medium. The vertical axis represents the common logarithm of cell number and the horizontal axis represents the time of culture in hours starting from the first sampling point. Triangles, Ax-2 wild-type cells; diamonds, qkgA^– strain1 C3; square, qkgA^– strain 2. The doubling time was calculated after curve fitting. (B) Cells feeding on bacterial lawns. Pre-cultured cells of the qkgA^– mutant clone QkgA-2 and wild-type control strain Ax-2 in HL-5 medium were harvested, washed in KK2 (16.5 mM KH₂PO₄, 3.8 mM K₂HPO₄, pH 6.2) and co-cultured with K.aerogenes on SM plates. After 5 days of incubation, the size of the plaques was measured.