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. 2003 Sep 15;31(18):e109. doi: 10.1093/nar/gng109

Figure 4.

Figure 4

Summary of the CVs for the pixel intensities and spot sizes and the variation with time and different pin sizes. (A) Average CVs of the signal intensities derived from 24 ORFs spotted in the indicated solution on two different days (series 1 and 2). Each bar represents the average CV measured from four replicates of each ORF and on two replicate slides (n = 192). The standard deviation CVs for each mean CV are represented by error bars. Series 1 corresponds to data obtained from freshly spotted slides and Series 2 corresponds to data obtained from slides spotted after storing the source plates at 4°C for 4 months. (B) CVs of spot sizes corresponding to Series 1 from (A) (black), to Series 2 (gray) and to spots printed with smaller pins (150 µm) at the same time as Series 2 (white).